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DHX16 Polyclonal antibody

DHX16 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11021-1-AP

Synonyms

ATP dependent RNA helicase #3, DBP2, DDX16, DEAH box protein 16, DHX16, KIAA0577, PRO2014, PRP8

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of Jurkat using 11021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of HeLa using 11021-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of mouse testis using 11021-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DHX16 Polyclonal antibody (11021-1-AP)

    WB analysis of human brain using 11021-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DHX16 Polyclonal antibody (11021-1-AP)

    IHC staining of human lymphoma using 11021-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11021-1-AP (DHX16 antibody) at dilution of 1:10 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DHX16 Polyclonal antibody (11021-1-AP)

    IF Staining of MCF-7 using 11021-1-AP

    Immunofluorescent analysis of MCF-7 cells, using DHX16 antibody 11021-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, human brain tissue, mouse testis tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11021-1-AP targets DHX16 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX16 fusion protein Ag1482
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 16
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 119 kDa
GenBank Accession NumberBC009392
Gene Symbol DHX16
Gene ID (NCBI) 8449
RRIDAB_2245938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DHX16 antibody 11021-1-APDownload protocol
IHC protocol for DHX16 antibody 11021-1-APDownload protocol
IF protocol for DHX16 antibody 11021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols