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DHX29 Polyclonal antibody

DHX29 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13923-1-AP

Synonyms

DDX29, DEAH box protein 29, DHX29, Nucleic acid helicase DDXx

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of HEK-293 using 13923-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of K-562 using 13923-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of PC-3 using 13923-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of HT-1080 using 13923-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of MCF-7 using 13923-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using DHX29 Polyclonal antibody (13923-1-AP)

    WB analysis of HepG2 using 13923-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HT-1080 cells, K-562 cells, MCF-7 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13923-1-AP targets DHX29 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX29 fusion protein Ag4938
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 29
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 150-155 kDa
GenBank Accession NumberBC056219
Gene Symbol DHX29
Gene ID (NCBI) 54505
RRIDAB_2092005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DHX29 antibody 13923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Lilas (Verified Customer) (06-29-2021)

Used antibody at a 1:1000 dilution in 5% BSA, and overnight incubation at 4 degrees. A very sharp band detected at around 155 kDA using western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma cell lines