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  • Featured Product
  • KD/KO Validated

DIAPH3 Polyclonal antibody

DIAPH3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14342-1-AP

Synonyms

diap3, DIAPH3, Diaphanous related formin 3, DRF3, mDia2, Protein diaphanous homolog 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HeLa using 14342-1-AP

    WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HeLa using 14342-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HEK-293 using 14342-1-AP

    WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.

  • Western Blot (WB) analysis of DU 145 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of DU 145 using 14342-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    WB analysis of HEK-293 using 14342-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IP experiment of HeLa using 14342-1-AP

    IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human cervical cancer using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of mouse testis using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of rat testis using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human cervical cancer using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human prostate cancer using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DIAPH3 Polyclonal antibody (14342-1-AP)

    IHC staining of human prostate cancer using 14342-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IF Staining of HeLa using 14342-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIAPH3 Polyclonal antibody (14342-1-AP)

    IF Staining of HeLa using 14342-1-AP

    Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, DU 145 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human cervical cancer tissue, rat testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14342-1-AP targets DIAPH3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIAPH3 fusion protein Ag5656
Full Name diaphanous homolog 3 (Drosophila)
Calculated Molecular Weight 137 kDa
Observed Molecular Weight 125-137 kDa
GenBank Accession NumberBC048963
Gene Symbol DIAPH3
Gene ID (NCBI) 81624
RRIDAB_2092930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

Protocols

Product Specific Protocols
WB protocol for DIAPH3 antibody 14342-1-APDownload protocol
IHC protocol for DIAPH3 antibody 14342-1-APDownload protocol
IF protocol for DIAPH3 antibody 14342-1-APDownload protocol
IP protocol for DIAPH3 antibody 14342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIP

Blood

Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.

Authors - Yijie Liu
  • KO Validated
View Article
humanIF

Sci Adv

Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.

Authors - Amar J Majmundar
View Article
humanIHC,WB

Aging (Albany NY)

The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer

Authors - Xiaowei Chen
View Article
humanWB

Cell Res

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

Authors - Stastna Jana J
  • KD Validated
View Article
humanWB,IHC,IF

EMBO J

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang
View Article
humanWB

Elife

Generation of stress fibers through myosin-driven reorganization of the actin cortex

Authors - Jaakko I Lehtimäki
  • KD Validated
View Article