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DIS3L2 Polyclonal antibody

DIS3L2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25792-1-AP

Synonyms

DIS3 like exonuclease 2, DIS3L2, FAM6A

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using DIS3L2 Polyclonal antibody (25792-1-AP)

    WB analysis of HepG2 using 25792-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25792-1-AP (DIS3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse embryo tissue using DIS3L2 Polyclonal antibody (25792-1-AP)

    WB analysis of mouse embryo using 25792-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 25792-1-AP (DIS3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using DIS3L2 Polyclonal antibody (25792-1-AP)

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DIS3L2 Polyclonal antibody (25792-1-AP)

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, mouse embryo tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25792-1-AP targets DIS3L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIS3L2 fusion protein Ag22963
Full Name DIS3 mitotic control homolog (S. cerevisiae)-like 2
Calculated Molecular Weight 885 aa, 99 kDa
Observed Molecular Weight 65 kDa, 99 kda
GenBank Accession NumberBC030113
Gene Symbol DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2880240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

Protocols

Product Specific Protocols
WB protocol for DIS3L2 antibody 25792-1-APDownload protocol
IHC protocol for DIS3L2 antibody 25792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article