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DIS3L2 Monoclonal antibody

DIS3L2 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9F2

Cat no : 67623-1-Ig

Synonyms

DIS3 like exonuclease 2, DIS3L2, FAM6A, FLJ36974

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DIS3L2 Monoclonal antibody (67623-1-Ig)

    WB analysis using 67623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using DIS3L2 Monoclonal antibody (67623-1-Ig)

    WB analysis using 67623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Flow cytometry (FC) experiment of Jurkat cells using DIS3L2 Monoclonal antibody (67623-1-Ig)

    FC experiment of Jurkat using 67623-1-Ig

    1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human DIS3L2 (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inU2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67623-1-Ig targets DIS3L2 in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DIS3L2 fusion protein Ag29992
Full Name DIS3 mitotic control homolog (S. cerevisiae)-like 2
Calculated Molecular Weight 885 aa, 99 kDa
Observed Molecular Weight 65 kDa, 99 kda
GenBank Accession NumberBC030113
Gene Symbol DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2882825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

Protocols

Product Specific Protocols
WB protocol for DIS3L2 antibody 67623-1-IgDownload protocol
FC protocol for DIS3L2 antibody 67623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential

Authors - Damian M Janecki
View Article