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TID1 Polyclonal antibody

TID1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11088-1-AP

Synonyms

DnaJ protein Tid 1, DNAJA3, HCA57, hTid 1, TID 1, TID1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TID1 Polyclonal antibody (11088-1-AP)

    WB analysis of Jurkat using 11088-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TID1 Polyclonal antibody (11088-1-AP)

    WB analysis of K-562 using 11088-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TID1 Polyclonal antibody (11088-1-AP)

    WB analysis of Jurkat using 11088-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11088-1-AP (TID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using TID1 Polyclonal antibody (11088-1-AP)

    IP experiment of K-562 using 11088-1-AP

    IP result of anti-TID1 (IP:11088-1-AP, 4ug; Detection:11088-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TID1 Polyclonal antibody (11088-1-AP)

    IHC staining of human lung cancer using 11088-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11088-1-AP (TID1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TID1 Polyclonal antibody (11088-1-AP)

    IHC staining of human pancreas cancer using 11088-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11088-1-AP (TID1 antibody) at dilution of 1:100 (under 25x lens).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11088-1-AP targets TID1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TID1 fusion protein Ag1558
Full Name DnaJ (Hsp40) homolog, subfamily A, member 3
Calculated Molecular Weight 480 aa, 53 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC011855
Gene Symbol DNAJA3
Gene ID (NCBI) 9093
RRIDAB_2293102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TID1 antibody 11088-1-APDownload protocol
IHC protocol for TID1 antibody 11088-1-APDownload protocol
IP protocol for TID1 antibody 11088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Role of human HSPE1 for OPA1 processing independent of HSPD1

Authors - Nelson Yeung
View Article