- Featured Product
- KD/KO Validated
DNM3 Polyclonal antibody
DNM3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14737-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
Product Information
14737-1-AP targets DNM3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DNM3 fusion protein Ag6381 |
| Full Name | dynamin 3 |
| Calculated Molecular Weight | 98 kDa |
| Observed Molecular Weight | 97-100 kDa |
| GenBank Accession Number | BC064546 |
| Gene Symbol | DNM3 |
| Gene ID (NCBI) | 26052 |
| RRID | AB_2093691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IHC protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IF protocol for DNM3 antibody 14737-1-AP | Download protocol |
| IP protocol for DNM3 antibody 14737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals | ||
Mol Cell Proteomics Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals. | ||
J Biol Chem Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa. | ||
Mol Hum Reprod Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis. | ||
Clin Exp Metastasis Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.
| ||
Biol Reprod Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. |
