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DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for FC, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 17382-1-AP

Synonyms

DNA nucleotidylexotransferase, DNTT, TDT, Terminal addition enzyme, Terminal transferase

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of Raji cells using DNTT,TDT Polyclonal antibody (17382-1-AP)

    IP experiment of Raji using 17382-1-AP

    IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DNTT,TDT Polyclonal antibody (17382-1-AP)

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DNTT,TDT Polyclonal antibody (17382-1-AP)

    IHC staining of human lymphoma using 17382-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of Jurkat cells using DNTT,TDT Polyclonal antibody (17382-1-AP)

    FC experiment of Jurkat using 17382-1-AP

    1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human DNTT,TDT (17382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17382-1-AP is DNTT,TDT Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNTT,TDT fusion protein Ag11235
Full Name deoxynucleotidyltransferase, terminal
Calculated Molecular Weight 509 aa, 58 kDa
GenBank Accession NumberBC012920
Gene Symbol DNTT
Gene ID (NCBI) 1791
RRIDAB_2878399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
IP protocol for DNTT,TDT antibody 17382-1-APDownload protocol
FC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols