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DOC2A Polyclonal antibody

DOC2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20575-1-AP

Synonyms

Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOC2A Polyclonal antibody (20575-1-AP)

    WB analysis using 20575-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20575-1-AP (DOC2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DOC2A Polyclonal antibody (20575-1-AP)

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DOC2A Polyclonal antibody (20575-1-AP)

    IHC staining of mouse brain using 20575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20575-1-AP targets DOC2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name double C2-like domains, alpha
Calculated Molecular Weight 44 kDa
Observed Molecular Weight18-44 kDa
GenBank Accession NumberNM_003586
Gene Symbol DOC2A
Gene ID (NCBI) 8448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOC2, Double C2-like domain-containing protein alpha, is involved in Ca(2+)-dependent neurotransmitter release. DOC2A and DOC2B are sensors for neuronal activity with unique calcium-dependent and kinetic properties (PMID: 16515538). DOC2A is mainly expressed in brain and also expressed in testis (PMID: 7826360).

Protocols

Product Specific Protocols
WB protocol for DOC2A antibody 20575-1-APDownload protocol
IHC protocol for DOC2A antibody 20575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols