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DOCK180 Polyclonal antibody

DOCK180 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23421-1-AP

Synonyms

ced5, dedicator of cytokinesis 1, DOCK1, DOCK180

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of HEK-293 using 23421-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of A431 using 23421-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of LNCaP using 23421-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of HeLa using 23421-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DOCK180 Polyclonal antibody (23421-1-AP)

    WB analysis of MCF-7 using 23421-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    IP experiment of HeLa using 23421-1-AP

    IP result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK180 Polyclonal antibody (23421-1-AP)

    IHC staining of human colon using 23421-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using DOCK180 Polyclonal antibody (23421-1-AP)

    IHC staining of human colon using 23421-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DOCK180 Polyclonal antibody (23421-1-AP)

    IF Staining of HeLa using 23421-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 23421-1-AP is DOCK180 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivitymouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK180 fusion protein Ag18069
Full Name dedicator of cytokinesis 1
Calculated Molecular Weight 1865 aa, 215 kDa
Observed Molecular Weight 215 kDa
GenBank Accession NumberBC146857
Gene Symbol DOCK1
Gene ID (NCBI) 1793
RRIDAB_2879276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DOCK180 antibody 23421-1-APDownload protocol
IHC protocol for DOCK180 antibody 23421-1-APDownload protocol
IF protocol for DOCK180 antibody 23421-1-APDownload protocol
IP protocol for DOCK180 antibody 23421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.

Authors - Hua Qu
View Article
mouseWB

Cell Death Dis

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Authors - Xujuan Sun
View Article
zebrafishWB

bioRxiv

Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin

Authors - Ryan A Doan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

k. (Verified Customer) (10-26-2023)

I used it for WB with 5% BSA+TBST at 4°C overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1;500
  • Cell Tissue Type: Human Liver cells