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DOCK1 Monoclonal antibody

DOCK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C3F12

Cat no : 67296-1-Ig

Synonyms

ced5, dedicator of cytokinesis 1, DOCK1, DOCK180

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK1 Monoclonal antibody (67296-1-Ig)

    WB analysis using 67296-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67296-1-Ig (DOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DOCK1 Monoclonal antibody (67296-1-Ig)

    WB analysis using 67296-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67296-1-Ig (DOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, 4T1 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67296-1-Ig targets DOCK1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DOCK1 fusion protein Ag18069
Full Name dedicator of cytokinesis 1
Calculated Molecular Weight 1865 aa, 215 kDa
Observed Molecular Weight 215 kDa
GenBank Accession NumberBC146857
Gene Symbol DOCK1
Gene ID (NCBI) 1793
RRIDAB_2882560
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK1, also named as DOCK180, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. DOCK1 functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1

Protocols

Product Specific Protocols
WB protocol for DOCK1 antibody 67296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols