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DOK5 Polyclonal antibody

DOK5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11948-1-AP

Synonyms

C20orf180, docking protein 5, DOK5, IRS 6, IRS6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using DOK5 Polyclonal antibody (11948-1-AP)

    WB analysis of mouse liver using 11948-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11948-1-AP (DOK5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11948-1-AP targets DOK5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK5 fusion protein Ag2615
Full Name docking protein 5
Calculated Molecular Weight 306 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC008992
Gene Symbol DOK5
Gene ID (NCBI) 55816
RRIDAB_10665550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOK5 belongs to the docking family of proteins that contain tandem pleckstrin homology-phosphotyrosine binding (PH-PTB) domains at their amino termini (PMID: 19909370). DOK proteins are also known as INS receptor substrates (IRS) and serve as substrates for INS and INS-like growth factor (PMID: 12730241 ) as well as various protein tyrosine kinases (PMID: 16647839). DOK5 functions as a specific signal transduction molecule and plays an important role in cellular differentiation. DOK5 is upregulated in systemic Sclerosis and Associated with IGFBP-5-Induced fibrosis (PMID: 24551065).

Protocols

Product Specific Protocols
WB protocol for DOK5 antibody 11948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols