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DPY30 Polyclonal antibody

DPY30 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16281-1-AP

Synonyms

dpy 30 homolog (C. elegans), Dpy 30 like protein, Dpy 30L, DPY30, HDPY 30, Protein dpy 30 homolog, Saf19

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DPY30 Polyclonal antibody (16281-1-AP)

    IHC staining of human stomach cancer using 16281-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DPY30 Polyclonal antibody (16281-1-AP)

    IHC staining of human stomach cancer using 16281-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DPY30 Polyclonal antibody (16281-1-AP)

    IHC staining of human ovary tumor using 16281-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16281-1-AP (DPY30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DPY30 Polyclonal antibody (16281-1-AP)

    IF Staining of A431 using 16281-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DPY30 antibody (16281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inhuman stomach cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16281-1-AP targets DPY30 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPY30 fusion protein Ag9370
Full Name dpy-30 homolog (C. elegans)
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC015970
Gene Symbol DPY30
Gene ID (NCBI) 84661
RRIDAB_2918044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for DPY30 antibody 16281-1-APDownload protocol
IF protocol for DPY30 antibody 16281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Biol

A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance

Authors - Jiaojiao Hao
View Article