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- KD/KO Validated
DRG2 Polyclonal antibody
DRG2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14743-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, SMMC-7721 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 9 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
14743-1-AP targets DRG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DRG2 fusion protein Ag6433 |
| Full Name | developmentally regulated GTP binding protein 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41-45 kDa |
| GenBank Accession Number | BC000493 |
| Gene Symbol | DRG2 |
| Gene ID (NCBI) | 1819 |
| RRID | AB_2230800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IHC protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IF protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IP protocol for DRG2 antibody 14743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. | ||
Int J Mol Sci DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial Dysfunction.
| ||
Oxid Med Cell Longev DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts
| ||
FASEB J Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain. | ||
Mol Cell Endocrinol RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation. | ||
Biochem Biophys Res Commun Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1.
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