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  • KD/KO Validated

DTX3 Polyclonal antibody

DTX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25304-1-AP

Synonyms

deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154



Tested Applications

Positive WB detected inmouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTX3 fusion protein Ag18111
Full Name deltex homolog 3 (Drosophila)
Calculated Molecular Weight 347 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC114498
Gene Symbol DTX3
Gene ID (NCBI) 196403
RRIDAB_2880021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.

Protocols

Product Specific Protocols
WB protocol for DTX3 antibody 25304-1-APDownload protocol
IHC protocol for DTX3 antibody 25304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.

Authors - Lidong Wang
  • KD Validated
humanWB

Mol Biol Rep

Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.

Authors - Hongli Xu
  • KO Validated