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DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A5

Cat no : 67672-1-Ig

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DVL1 Monoclonal antibody (67672-1-Ig)

    WB analysis using 67672-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using DVL1 Monoclonal antibody (67672-1-Ig)

    WB analysis of ROS1728 using 67672-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using DVL1 Monoclonal antibody (67672-1-Ig)

    WB analysis of NCCIT using 67672-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67672-1-Ig (DVL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL1 Monoclonal antibody (67672-1-Ig)

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using DVL1 Monoclonal antibody (67672-1-Ig)

    IHC staining of rat colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL1 Monoclonal antibody (67672-1-Ig)

    IHC staining of mouse colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using DVL1 Monoclonal antibody (67672-1-Ig)

    IHC staining of mouse colon using 67672-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67672-1-Ig (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67672-1-Ig targets DVL1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DVL1 fusion protein Ag26083
Full Name dishevelled, dsh homolog 1 (Drosophila)
Calculated Molecular Weight 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
RRIDAB_2882868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for DVL1 antibody 67672-1-IgDownload protocol
IHC protocol for DVL1 antibody 67672-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols