DVL3 Polyclonal antibody

DVL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13444-1-AP

Synonyms

Dishevelled 3, DSH homolog 3, DVL3, KIAA0208



Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue, mouse colon tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13444-1-AP targets DVL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DVL3 fusion protein Ag4272
Full Name dishevelled, dsh homolog 3 (Drosophila)
Calculated Molecular Weight 716 aa, 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC032459
Gene Symbol DVL3
Gene ID (NCBI) 1857
RRIDAB_2093451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DVL3 antibody 13444-1-APDownload protocol
IHC protocol for DVL3 antibody 13444-1-APDownload protocol
IF protocol for DVL3 antibody 13444-1-APDownload protocol
IP protocol for DVL3 antibody 13444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusWB,IP

J Cell Biol

Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.

Authors - Moonsup Lee
mouseIF

J Cell Biol

Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.

Authors - Andre Landin Malt
humanWB

Oncogene

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

Authors - Kai Li
humanWB

Cancer Lett

SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.

Authors - Li Xuefei X
humanWB

Oncotarget

Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells.

Authors - Xiaohui Zhu
humanWB,IHC,IF

Biol Proced Online

Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3

Authors - Mengqi Zhu