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Dermatopontin Monoclonal antibody

Dermatopontin Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B1A12

Cat no : 66348-1-Ig

Synonyms

dermatopontin, DPT, TRAMP

Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Dermatopontin Monoclonal antibody (66348-1-Ig)

    WB analysis of rat skin using 66348-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66348-1-Ig (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using Dermatopontin Monoclonal antibody (66348-1-Ig)

    WB analysis of human testis using 66348-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66348-1-Ig (Dermatopontin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Dermatopontin Monoclonal antibody (66348-1-Ig)

    IF Staining of SH-SY5Y using 66348-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Dermatopontin antibody (66348-1-Ig, Clone: 4B1A12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inrat skin tissue, human testis tissue
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66348-1-Ig targets Dermatopontin in WB, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Dermatopontin fusion protein Ag6200
Full Name dermatopontin
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC033736
Gene Symbol DPT
Gene ID (NCBI) 1805
RRIDAB_2881728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the inhibition activity of TGF-beta1.

Protocols

Product Specific Protocols
WB protocol for Dermatopontin antibody 66348-1-IgDownload protocol
IF protocol for Dermatopontin antibody 66348-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols