Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25770-1-AP

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells, K-562 cells, Neuro-2a cells, Jurkat cells
Positive IF detected inMCF-7 cells, SH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25770-1-AP targets Drebrin in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Drebrin fusion protein Ag22634
Full Name drebrin 1
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC007567
Gene Symbol DBN1
Gene ID (NCBI) 1627
RRIDAB_2880234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 25770-1-APDownload protocol
IF protocol for Drebrin antibody 25770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Authors - Paloma Alvarez-Suarez