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EARS2 Polyclonal antibody

EARS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17539-1-AP

Synonyms

EARS2, GluRS, Glutamate tRNA ligase, KIAA1970, MSE1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver mitochondria tissue using EARS2 Polyclonal antibody (17539-1-AP)

    WB analysis of mouse liver mitochondria using 17539-1-AP

    mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 17539-1-AP (EARS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver mitochondria tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17539-1-AP targets EARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EARS2 fusion protein Ag11594
Full Name glutamyl-tRNA synthetase 2, mitochondrial (putative)
Calculated Molecular Weight 534 aa, 60 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC040013
Gene Symbol EARS2
Gene ID (NCBI) 124454
RRIDAB_2878414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EARS2 encodes mitochondrial glutamyltRNA synthetase 2. Two isoforms were produced by alternative splicing with predicted MW of 58 and 60 kDa according to UniProt, and this antibody recognises both.

Protocols

Product Specific Protocols
WB protocol for EARS2 antibody 17539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols