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ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26088-1-AP

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis using 26088-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis of A549 using 26088-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis of HeLa using 26088-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECE1 Polyclonal antibody (26088-1-AP)

    IHC staining of human breast cancer using 26088-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECE1 Polyclonal antibody (26088-1-AP)

    IHC staining of human breast cancer using 26088-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26088-1-AP is ECE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECE1 fusion protein Ag23144
Full Name endothelin converting enzyme 1
Observed Molecular Weight 125-135 kDa
GenBank Accession NumberBC126257
Gene Symbol ECE1
Gene ID (NCBI) 1889
RRIDAB_2880371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ECE1 antibody 26088-1-APDownload protocol
IHC protocol for ECE1 antibody 26088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jaouhara (Verified Customer) (12-05-2023)

Specific antibody. Validated in immunofluorescence and immunohistochemistry. Good signal.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Cell Tissue Type: Chondrocytes