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ECHDC1 Polyclonal antibody

ECHDC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27732-1-AP

Synonyms

ECHDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using ECHDC1 Polyclonal antibody (27732-1-AP)

    WB analysis of PC-12 using 27732-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of A549 cells using ECHDC1 Polyclonal antibody (27732-1-AP)

    WB analysis of A549 using 27732-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using ECHDC1 Polyclonal antibody (27732-1-AP)

    WB analysis of PC-12 using 27732-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ECHDC1 Polyclonal antibody (27732-1-AP)

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ECHDC1 Polyclonal antibody (27732-1-AP)

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ECHDC1 Polyclonal antibody (27732-1-AP)

    IHC staining of human lung cancer using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ECHDC1 Polyclonal antibody (27732-1-AP)

    IHC staining of human lung cancer using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-12 cells, A549 cells
Positive IHC detected inmouse kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27732-1-AP targets ECHDC1 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECHDC1 fusion protein Ag26905
Full Name enoyl Coenzyme A hydratase domain containing 1
Calculated Molecular Weight 307 aa, 34 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC003549
Gene Symbol ECHDC1
Gene ID (NCBI) 55862
RRIDAB_2880956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ECHDC1 antibody 27732-1-APDownload protocol
IHC protocol for ECHDC1 antibody 27732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols