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ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

6A12B3

Cat no : 66023-1-Ig

Synonyms

ECM1, extracellular matrix protein 1, Secretory component p85

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ECM1 Monoclonal antibody (66023-1-Ig)

    WB analysis of MCF-7 using 66023-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ECM1 Monoclonal antibody (66023-1-Ig)

    WB analysis of human heart using 66023-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ECM1 Monoclonal antibody (66023-1-Ig)

    WB analysis of COLO 320 using 66023-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECM1 Monoclonal antibody (66023-1-Ig)

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECM1 Monoclonal antibody (66023-1-Ig)

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECM1 Monoclonal antibody (66023-1-Ig)

    IHC staining of human liver cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECM1 Monoclonal antibody (66023-1-Ig)

    IHC staining of human liver cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inMCF7 cells, COLO 320 cells, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66023-1-Ig is ECM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ECM1 fusion protein Ag17955
Full Name extracellular matrix protein 1
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.