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  • Featured Product
  • KD/KO Validated

ECOP Polyclonal antibody

ECOP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12611-1-AP

Synonyms

ECOP, GASP, VOPP1

Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using ECOP Polyclonal antibody (12611-1-AP)

    WB analysis of Transfected HEK-293 using 12611-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12611-1-AP (ECOP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ECOP Polyclonal antibody (12611-1-AP)

    IHC staining of human kidney using 12611-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12611-1-AP (ECOP Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ECOP Polyclonal antibody (12611-1-AP)

    IHC staining of mouse ovary using 12611-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12611-1-AP (ECOP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ECOP Polyclonal antibody (12611-1-AP)

    IHC staining of mouse ovary using 12611-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12611-1-AP (ECOP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ECOP Polyclonal antibody (12611-1-AP)

    IHC staining of human kidney using 12611-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12611-1-AP (ECOP Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12611-1-AP targets ECOP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECOP fusion protein Ag3225
Full Name EGFR-coamplified and overexpressed protein
Calculated Molecular Weight 172 aa, 19 kDa
GenBank Accession NumberBC016650
Gene Symbol ECOP
Gene ID (NCBI) 81552
RRIDAB_2877866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ECOP, also named as VOPP1 and GASP, has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. It interacts directly with cytoplasmic mediators of the NF kappa B pathway. ECOP has three isoforms with MW 18-19 kDa.

Protocols

Product Specific Protocols
WB protocol for ECOP antibody 12611-1-APDownload protocol
IHC protocol for ECOP antibody 12611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

BMC Biol

VOPP1 promotes breast tumorigenesis by interacting with the tumor suppressor WWOX.

Authors - Florian Bonin
  • KD Validated
View Article
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
View Article