EEF1D Antibody 0 Publications

Mouse Monoclonal| Catalog number: 60085-2-Ig |CloneNo.: 3G4C4

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Freight/Packing

Con: 150 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
transfection MCF-7 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:
human pancreas cancer tissue

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Antigen affinity purification

Isotype:
IgG1

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)

Calculated molecular weight:
31 kDa

Observed molecular weight:
35-40 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
EEF1D

Synonyms
Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FLJ20897, FP1047
Background

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )


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