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KD/KO Validated

Ephrin A3 Polyclonal antibody

Name: Ephrin A3 Polyclonal antibody
Brand: Proteintech
SKU: 12480-1-AP

Ephrin A3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12480-1-AP

Synonyms

EFL 2, EFL2, EFNA3, Ehk1 L, EHK1 ligand, ephrin A3, EPLG3, LERK 3, LERK3

Validation Data Gallery

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AllWBIPIHCFC

WB analysis using 12480-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12480-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12480-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12480-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 12480-1-AP
IP result of anti-Ephrin A3 (IP:12480-1-AP, 4ug; Detection:12480-1-AP 1:500) with A549 cells lysate 1040ug.
IHC staining of human skeletal muscle using 12480-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 12480-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 40x lens).
FC experiment of Jurkat using 12480-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Ephrin A3 (12480-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 12480-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, A549 cells, rat brain tissue
Positive IP detected in	A549 cells
Positive IHC detected in	human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

12480-1-AP targets Ephrin A3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Ephrin A3 fusion protein Ag3188
Full Name	ephrin-A3
Calculated Molecular Weight	238 aa, 26 kDa
Observed Molecular Weight	26-35 kDa
GenBank Accession Number	BC017722
Gene Symbol	EFNA3
Gene ID (NCBI)	1944
RRID	AB_10638305
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Ephrin A3 antibody 12480-1-AP	Download protocol
IHC protocol for Ephrin A3 antibody 12480-1-AP	Download protocol
IP protocol for Ephrin A3 antibody 12480-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Med EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma. Authors - Ruzetuoheti Yiminniyaze View Article
human	WB	Biomed Res Int OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway. Authors - Hui Wang KD Validated View Article
human	WB,IF	J Exp Clin Cancer Res The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells Authors - Marianna Talia KD Validated View Article
rat	WB	Brain Res Bull Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats Authors - Tao Tong View Article

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Ephrin A3 Polyclonal antibody

Ephrin A3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 12480-1-AP

WB analysis of human brain using 12480-1-AP

WB analysis of A549 using 12480-1-AP

WB analysis of human brain using 12480-1-AP

IP experiment of A549 using 12480-1-AP

IHC staining of human skeletal muscle using 12480-1-AP

IHC staining of human skeletal muscle using 12480-1-AP

FC experiment of Jurkat using 12480-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma.

OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.

The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats