EIF1 Polyclonal antibody

EIF1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15276-1-AP

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15276-1-AP targets EIF1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1 fusion protein Ag7394
Full Name eukaryotic translation initiation factor 1
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC005118
Gene Symbol EIF1
Gene ID (NCBI) 10209
RRIDAB_2878121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

Protocols

Product Specific Protocols
WB protocol for EIF1 antibody 15276-1-APDownload protocol
IHC protocol for EIF1 antibody 15276-1-APDownload protocol
IP protocol for EIF1 antibody 15276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder

Authors - Huan Feng
WB

PLoS Pathog

SARS-CoV-2 Nsp1 cooperates with initiation factors EIF1 and 1A to selectively enhance translation of viral RNA

Authors - Ranen Aviner
monkeyWB

bioRxiv

SARS-CoV-2 Nsp1 regulates translation start site fidelity to promote infection

Authors - Ranen Aviner