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EIF1AX Polyclonal antibody

EIF1AX Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11649-2-AP

Synonyms

eIF 1A, eIF 1A X isoform, eIF 4C, EIF1A, EIF1AP1, EIF1AX, EIF4C

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EIF1AX Polyclonal antibody (11649-2-AP)

    WB analysis of HeLa using 11649-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF1AX Polyclonal antibody (11649-2-AP)

    WB analysis using 11649-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using EIF1AX Polyclonal antibody (11649-2-AP)

    IHC staining of human gliomas using 11649-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11649-2-AP (EIF1AX antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF1AX Polyclonal antibody (11649-2-AP)

    IF Staining of HeLa using 11649-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF1AX antibody (11649-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, HeLa cells, HEK-293T cells, Jurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11649-2-AP targets EIF1AX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1AX fusion protein Ag2248
Full Name eukaryotic translation initiation factor 1A, X-linked
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000793
Gene Symbol EIF1AX
Gene ID (NCBI) 1964
RRIDAB_2097803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

Protocols

Product Specific Protocols
WB protocol for EIF1AX antibody 11649-2-APDownload protocol
IHC protocol for EIF1AX antibody 11649-2-APDownload protocol
IF protocol for EIF1AX antibody 11649-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Prolif

Transcriptional repression of p21 by EIF1AX promotes the proliferation of breast cancer cells.

Authors - Yuhuan Li
View Article