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EIF2AK1 Monoclonal antibody

EIF2AK1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H1F3

Cat no : 67674-1-Ig

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    WB analysis using 67674-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    WB analysis using 67674-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2AK1 Monoclonal antibody (67674-1-Ig)

    IF Staining of HeLa using 67674-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67674-1-Ig targets EIF2AK1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2AK1 fusion protein Ag16970
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_2882869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Protocols

Product Specific Protocols
WB protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IHC protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IF protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols