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  • Featured Product
  • KD/KO Validated

eIF4G2/DAP5 Monoclonal antibody

eIF4G2/DAP5 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C11A3

Cat no : 67428-1-Ig

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, FLJ41344, NAT1, p97

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using human eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    WB analysis using 67428-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (EIF4G2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using human eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    WB analysis using 67428-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human breast cancer using 67428-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human breast cancer using 67428-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IF Staining of HepG2 using 67428-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IF Staining of HeLa using 67428-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using eIF4G2/DAP5 antibody (67428-1-Ig, Clone: 3C11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67428-1-Ig targets eIF4G2/DAP5 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12080
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2882667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for eIF4G2/DAP5 antibody 67428-1-IgDownload protocol
IHC protocol for eIF4G2/DAP5 antibody 67428-1-IgDownload protocol
IF protocol for eIF4G2/DAP5 antibody 67428-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell

ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

Authors - Yichen Xu
  • KD Validated
View Article