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ELAC1 Polyclonal antibody

ELAC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26639-1-AP

Synonyms

D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ELAC1 Polyclonal antibody (26639-1-AP)

    WB analysis of mouse liver using 26639-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26639-1-AP (ELAC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ELAC1 Polyclonal antibody (26639-1-AP)

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ELAC1 Polyclonal antibody (26639-1-AP)

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26639-1-AP is ELAC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELAC1 fusion protein Ag23939
Full Name elaC homolog 1 (E. coli)
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC014624
Gene Symbol ELAC1
Gene ID (NCBI) 55520
RRIDAB_2880586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ELAC1 antibody 26639-1-APDownload protocol
IHC protocol for ELAC1 antibody 26639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols