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ENSA Polyclonal antibody

ENSA Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14518-1-AP

Synonyms

Alpha endosulfine, ARPP 19e, endosulfine alpha, ENSA

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of mouse brain using 14518-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of HeLa using 14518-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human small intestine using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human small intestine using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    IF Staining of HeLa using 14518-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14518-1-AP (ENSA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14518-1-AP targets ENSA in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENSA fusion protein Ag5982
Full Name endosulfine alpha
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC004461
Gene Symbol ENSA
Gene ID (NCBI) 2029
RRIDAB_10838808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ENSA antibody 14518-1-APDownload protocol
IHC protocol for ENSA antibody 14518-1-APDownload protocol
IF protocol for ENSA antibody 14518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols