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CD39/ENTPD1 Monoclonal antibody

CD39/ENTPD1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2H6

Cat no : 60323-1-Ig

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    WB analysis using 60323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    WB analysis of Jurkat using 60323-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60323-1-Ig (ENTPD1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IHC staining of human placenta using 60323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IHC staining of human placenta using 60323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IF Staining of HeLa using 60323-1-Ig

    Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    FC experiment of Jurkat using 60323-1-Ig

    1X10^6 Jurkat cells were stained with 0.2ug ENTPD1 antibody (60323-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inJurkat cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60323-1-Ig targets CD39/ENTPD1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD39/ENTPD1 fusion protein Ag6165
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC047664
Gene Symbol ENTPD1
Gene ID (NCBI) 953
RRIDAB_2881434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.

Protocols

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
IHC protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
IF protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
FC protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols