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ENTPD2 Polyclonal antibody

ENTPD2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13656-1-AP

Synonyms

CD39 antigen like 1, CD39L1, Ecto ATP diphosphohydrolase 2, Ecto ATPase 2, Ecto ATPDase 2, ENTPD2, NTPDase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using ENTPD2 Polyclonal antibody (13656-1-AP)

    WB analysis of human placenta using 13656-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13656-1-AP (ENTPD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ENTPD2 Polyclonal antibody (13656-1-AP)

    WB analysis using 13656-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13656-1-AP (ENTPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using ENTPD2 Polyclonal antibody (13656-1-AP)

    IP experiment of K-562 using 13656-1-AP

    IP result of anti-ENTPD2 (IP:13656-1-AP, 4ug; Detection:13656-1-AP 1:300) with K-562 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENTPD2 Polyclonal antibody (13656-1-AP)

    IHC staining of human small intestine using 13656-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENTPD2 Polyclonal antibody (13656-1-AP)

    IHC staining of human small intestine using 13656-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman placenta tissue, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13656-1-AP targets ENTPD2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENTPD2 fusion protein Ag4513
Full Name ectonucleoside triphosphate diphosphohydrolase 2
Calculated Molecular Weight 495 aa, 54 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC035738
Gene Symbol ENTPD2
Gene ID (NCBI) 954
RRIDAB_2877966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD2(Ectonucleoside triphosphate diphosphohydrolase 2) is also named as CD39L1, Ecto-ATPase 2 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ENTPD2 antibody 13656-1-APDownload protocol
IHC protocol for ENTPD2 antibody 13656-1-APDownload protocol
IP protocol for ENTPD2 antibody 13656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Am J Physiol Gastrointest Liver Physiol

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Authors - Moyuru Hayashi
View Article