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ENTPD3 Polyclonal antibody

ENTPD3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13021-1-AP

Synonyms

CD39 antigen like 3, CD39L3, Ecto apyrase 3, Ecto ATP diphosphohydrolase 3, Ecto ATPase 3, Ecto ATPDase 3, ENTPD3, HB6, NTPDase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ENTPD3 Polyclonal antibody (13021-1-AP)

    WB analysis of human brain using 13021-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ENTPD3 Polyclonal antibody (13021-1-AP)

    WB analysis of Jurkat using 13021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ENTPD3 Polyclonal antibody (13021-1-AP)

    WB analysis of Raji using 13021-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13021-1-AP (ENTPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ENTPD3 Polyclonal antibody (13021-1-AP)

    IF Staining of U2OS using 13021-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ENTPD3 antibody (13021-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, Jurkat cells, Raji cells
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13021-1-AP targets ENTPD3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENTPD3 fusion protein Ag4114
Full Name ectonucleoside triphosphate diphosphohydrolase 3
Calculated Molecular Weight 529 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC029869
Gene Symbol ENTPD3
Gene ID (NCBI) 956
RRIDAB_2100082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD3, also known as NTPDase3 or CD39L3, belongs to the GDA1/CD39 NTPase family. ENTPD3 has a threefold preference for the hydrolysis of ATP over ADP. ENTPD3 is expressed in adult brain, pancreas, spleen and prostate, but not in liver and peripheral blood leukocytes. ENTPD3 has 2 isoforms with the molecular mass of 51 and 59 kDa.

Protocols

Product Specific Protocols
WB protocol for ENTPD3 antibody 13021-1-APDownload protocol
IF protocol for ENTPD3 antibody 13021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice.

Authors - Qian Wang
View Article