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EPHA1-specific Polyclonal antibody

EPHA1-specific Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18698-1-AP

Synonyms

EPH, EPH receptor A1, EPHA1, Ephrin type A receptor 1, EPHT, EPHT1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using EPHA1-specific Polyclonal antibody (18698-1-AP)

    WB analysis of A549 using 18698-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18698-1-AP (EPHA1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EPHA1-specific Polyclonal antibody (18698-1-AP)

    WB analysis of HeLa using 18698-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18698-1-AP (EPHA1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EPHA1-specific Polyclonal antibody (18698-1-AP)

    IHC staining of human liver cancer using 18698-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EPHA1-specific Polyclonal antibody (18698-1-AP)

    IHC staining of human liver cancer using 18698-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EPHA1-specific Polyclonal antibody (18698-1-AP)

    IF Staining of A549 using 18698-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using EPHA1-specific Polyclonal antibody (18698-1-AP)

    FC experiment of HeLa using 18698-1-AP

    1X10^6 HeLa cells were stained with 0.2ug EPHA1-specific antibody (18698-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18698-1-AP targets EPHA1-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name EPH receptor A1
Calculated Molecular Weight 108 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC130291
Gene Symbol EPHA1
Gene ID (NCBI) 2041
RRIDAB_10597245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

Protocols

Product Specific Protocols
WB protocol for EPHA1-specific antibody 18698-1-APDownload protocol
IHC protocol for EPHA1-specific antibody 18698-1-APDownload protocol
IF protocol for EPHA1-specific antibody 18698-1-APDownload protocol
FC protocol for EPHA1-specific antibody 18698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols