Con: 69 μg/150 μl
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IF result of EPHA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21875-1-AP(EPHA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours
IP Result of anti-EPHA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 SH-SY5Y cells were stained with 0.2ug EPHA4 antibody (21875-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.