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ETFDH Polyclonal antibody

Name: ETFDH Polyclonal antibody
Brand: Proteintech
SKU: 11109-1-AP

ETFDH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11109-1-AP

Synonyms

ETF dehydrogenase, ETF QO, ETF ubiquinone oxidoreductase, ETFDH, ETFQO, MADD

Validation Data Gallery

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AllWBIPIHC

WB analysis using 11109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11109-1-AP
WB result of ETFDH antibody (11109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETFDH transfected HEK-293 cells.
WB analysis using 11109-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11109-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11109-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11109-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11109-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11109-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11109-1-AP
IP result of anti-ETFDH (IP:11109-1-AP, 3ug; Detection:11109-1-AP 1:500) with mouse brain tissue lysate 8000ug.
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11109-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11109-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, HEK-293 cells, rat brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human liver cancer tissue, human liver tissue, human kidney tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

11109-1-AP targets ETFDH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ETFDH fusion protein Ag1568
Full Name	electron-transferring-flavoprotein dehydrogenase
Calculated Molecular Weight	64 kDa
Observed Molecular Weight	64 kDa
GenBank Accession Number	BC011890
Gene Symbol	ETFDH
Gene ID (NCBI)	2110
RRID	AB_2231382
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) is also named as ETF-QO and belongs to the ETF-QO/fixC family.It is a 64-kDa monomer integrated in the inner mitochondrial membrane, con- tains one molecule of FAD and a 4Fe4S cluster(PMID:12815589).Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C).This antibody is speicific to ETFDH.

Protocols

Product Specific Protocols
WB protocol for ETFDH antibody 11109-1-AP	Download protocol
IHC protocol for ETFDH antibody 11109-1-AP	Download protocol
IP protocol for ETFDH antibody 11109-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Ann Neurol Both ETFDH mutations and FAD homeostasis disturbance are essential for developing riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency. Authors - Jingwen Xu View Article
mouse	WB	Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. Authors - Min-Zhi Peng View Article
human	WB	Hum Mol Genet Clinical, pathological and genetic features and follow-up of 110 patients with late-onset MADD: A single-center retrospective study. Authors - Bing Wen View Article
human	WB	J Neurol Neurosurg Psychiatry Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations. Authors - Wen Bing B View Article
human	WB,IF	Front Oncol Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells. Authors - Chen Sun View Article
human	WB	J Mol Med (Berl) Molecular analysis of 51 unrelated pedigrees with late-onset multiple acyl-CoA dehydrogenation deficiency (MADD) in southern China confirmed the most common ETFDH mutation and high carrier frequency of c.250G>A. Authors - Wang Zhi-Qiang ZQ View Article

ETFDH Polyclonal antibody

ETFDH Polyclonal Antibody for IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 11109-1-AP

WB analysis of HEK-293 using 11109-1-AP

WB analysis using 11109-1-AP

WB analysis of HEK-293 using 11109-1-AP

WB analysis of rat brain using 11109-1-AP

WB analysis of mouse skeletal muscle using 11109-1-AP

WB analysis of mouse brain using 11109-1-AP

WB analysis of mouse kidney using 11109-1-AP

IP experiment of mouse brain using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human kidney using 11109-1-AP

IHC staining of human kidney using 11109-1-AP

IHC staining of human liver using 11109-1-AP

IHC staining of human liver using 11109-1-AP

IHC staining of human prostate cancer using 11109-1-AP

IHC staining of human prostate cancer using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

IHC staining of human liver cancer using 11109-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Both ETFDH mutations and FAD homeostasis disturbance are essential for developing riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency.

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Clinical, pathological and genetic features and follow-up of 110 patients with late-onset MADD: A single-center retrospective study.

Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations.

Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells.

Molecular analysis of 51 unrelated pedigrees with late-onset multiple acyl-CoA dehydrogenation deficiency (MADD) in southern China confirmed the most common ETFDH mutation and high carrier frequency of c.250G>A.