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ETV5 Polyclonal antibody

ETV5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13011-1-AP

Synonyms

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, Raji cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13011-1-AP targets ETV5 in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ETV5 fusion protein Ag3708
Full Name ets variant 5
Calculated Molecular Weight 510 aa, 58 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC007333
Gene Symbol ETV5
Gene ID (NCBI) 2119
RRIDAB_2278092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

Protocols

Product Specific Protocols
WB protocol for ETV5 antibody 13011-1-APDownload protocol
IHC protocol for ETV5 antibody 13011-1-APDownload protocol
IF protocol for ETV5 antibody 13011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.

Authors - Jaehong Suh
humanChIP

Nat Genet

The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation.

Authors - Daniel S Kim
mouseWB

Nat Commun

A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility

Authors - Daiji Kiyozumi
mouseIF

Nat Commun

A critical role for NF2 and the Hippo pathway in branching morphogenesis.

Authors - Antoine Reginensi
mouseIF, ChIP

Proc Natl Acad Sci U S A

Transcription factor Etv5 is essential for the maintenance of alveolar type II cells.

Authors - Zhen Zhang
mouseIF

PLoS Biol

Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis.

Authors - Paul Riccio