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  • KD/KO Validated

EXOC5 Polyclonal antibody

EXOC5 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17593-1-AP

Synonyms

EXOC5, exocyst complex component 5, HSEC10, PRO1912, SEC10, SEC10L1, SEC10P



Tested Applications

Positive WB detected inmouse brain tissue, human adrenal gland tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17593-1-AP targets EXOC5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOC5 fusion protein Ag11524
Full Name exocyst complex component 5
Calculated Molecular Weight 708 aa, 82 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC041126
Gene Symbol EXOC5
Gene ID (NCBI) 10640
RRIDAB_2101582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.

Protocols

Product Specific Protocols
WB protocol for EXOC5 antibody 17593-1-APDownload protocol
IHC protocol for EXOC5 antibody 17593-1-APDownload protocol
IF protocol for EXOC5 antibody 17593-1-APDownload protocol
IP protocol for EXOC5 antibody 17593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.

Authors - Bodemann Brian O BO
  • KD Validated
mouseWB

Elife

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

Authors - Kunli Zhao
humanWB

Sci Rep

Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Authors - Isabel Saez
humanWB,IHC

Mol Cancer Res

EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.

Authors - Haojie Li
  • KD Validated
mouseIF

Cells

The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells

Authors - Nicole K. Nakamura
mouseWB

J Biol Chem

Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.

Authors - Brent A Fujimoto
  • KO Validated