EXTL3 Polyclonal antibody

EXTL3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10588-1-AP

Synonyms

botv, EXTL3, KIAA0519, REG, REGR, RPR



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10588-1-AP targets EXTL3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXTL3 fusion protein Ag0936
Full Name exostoses (multiple)-like 3
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC006363
Gene Symbol EXTL3
Gene ID (NCBI) 2137
RRIDAB_2262577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397).

Protocols

Product Specific Protocols
WB protocol for EXTL3 antibody 10588-1-APDownload protocol
IHC protocol for EXTL3 antibody 10588-1-APDownload protocol
IF protocol for EXTL3 antibody 10588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Endocr Pract

Discovery of a human peptide sequence signaling islet neogenesis.

Authors - Levetan Claresa S CS
human,mouseWB,IF

Ann N Y Acad Sci

REG1A protects retinal photoreceptors from blue light damage

Authors - Ze-Hua Xu