F11R Polyclonal antibody

F11R Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 26941-1-AP

Synonyms

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor



Tested Applications

Positive WB detected inHUVEC cells, human peripheral blood platelets, U-87 MG cells
Positive IHC detected inhuman breast cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26941-1-AP targets F11R in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F11R fusion protein Ag25641
Full Name F11 receptor
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001533
Gene Symbol F11R
Gene ID (NCBI) 50848
RRIDAB_2880692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

Protocols

Product Specific Protocols
WB protocol for F11R antibody 26941-1-APDownload protocol
IHC protocol for F11R antibody 26941-1-APDownload protocol
FC protocol for F11R antibody 26941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols