Factor XII Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66089-1-Ig |CloneNo.: 2E2F11

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Con: 192 μg/150 μl

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Species specificity:
human

Positive WB detected in:
K562 cells, K-562 cells

Positive IHC detected in:
human liver tissue, human kidney tissue, human liver tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
coagulation factor XII (Hageman factor)

Calculated molecular weight:
615aa,68 kDa

Observed molecular weight:
28 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
F12

Synonyms
Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor
Background

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. When plasma comes into contact with negatively charged surfaces such as glass, kaolin, or dextran sulfate, F XII is bound to the surface and undergoes limited proteolysis by plasma kallikrein, resulting in two active enzyme forms, ɑ-F XIIa and β-F XIIa. ɑ-F XIIa consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain. β-F XIIa consists of two disulfide-linked chains, a 2 kD light chain and a 28 kD heavy chain containing the catalytic domain. Defects in Factor XII do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.


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