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  • KD/KO Validated

FAAH Polyclonal antibody

FAAH Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17909-1-AP

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1



Tested Applications

Positive WB detected inmouse liver tissue, rat testis tissue, A431 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse testis tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17909-1-AP is FAAH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAAH fusion protein Ag12304
Full Name fatty acid amide hydrolase
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC093632
Gene Symbol FAAH
Gene ID (NCBI) 2166
RRIDAB_2878465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Protocols

Product Specific Protocols
WB protocol for FAAH antibody 17909-1-APDownload protocol
IHC protocol for FAAH antibody 17909-1-APDownload protocol
IP protocol for FAAH antibody 17909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.

Authors - Shuting Xia
ratWB

J Proteome Res

Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention.

Authors - Xiang Zhan
mouseWB

Neuroscience

The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.

Authors - Jinming Zhang
human,mouseWB,IP

Cell Death Differ

FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system

Authors - Yangyang Zhu
  • KO Validated
humanWB

Cell Death Dis

Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway

Authors - Junfeng Hao