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FAHD1 Polyclonal antibody

FAHD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26233-1-AP

Synonyms

C16orf36, FAHD1, YisK like protein, YISKL

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using FAHD1 Polyclonal antibody (26233-1-AP)

    WB analysis of mouse kidney using 26233-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26233-1-AP (FAHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAHD1 Polyclonal antibody (26233-1-AP)

    WB analysis of HeLa using 26233-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26233-1-AP (FAHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using FAHD1 Polyclonal antibody (26233-1-AP)

    IP experiment of mouse kidney using 26233-1-AP

    IP result of anti-FAHD1 (IP:26233-1-AP, 4ug; Detection:26233-1-AP 1:1000) with mouse kidney tissue lysate 4800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAHD1 Polyclonal antibody (26233-1-AP)

    IHC staining of human kidney using 26233-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26233-1-AP (FAHD1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAHD1 Polyclonal antibody (26233-1-AP)

    IHC staining of human kidney using 26233-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26233-1-AP (FAHD1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26233-1-AP targets FAHD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAHD1 fusion protein Ag23966
Full Name fumarylacetoacetate hydrolase domain containing 1
Observed Molecular Weight 27 Kda
GenBank Accession NumberBC063017
Gene Symbol FAHD1
Gene ID (NCBI) 81889
RRIDAB_2880436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAHD1 antibody 26233-1-APDownload protocol
IHC protocol for FAHD1 antibody 26233-1-APDownload protocol
IP protocol for FAHD1 antibody 26233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols