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FAM119A Polyclonal antibody

FAM119A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21079-1-AP

Synonyms

FAM119A, HCA557b, METTL21A, Protein FAM119A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM119A Polyclonal antibody (21079-1-AP)

    WB analysis using 21079-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21079-1-AP (FAM119A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FAM119A Polyclonal antibody (21079-1-AP)

    WB analysis of L02 using 21079-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21079-1-AP (FAM119A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using FAM119A Polyclonal antibody (21079-1-AP)

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FAM119A Polyclonal antibody (21079-1-AP)

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM119A Polyclonal antibody (21079-1-AP)

    IF Staining of HeLa using 21079-1-AP

    Immunofluorescent analysis of HepG2 cells, using FAM119A antibody 21079-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM119A Polyclonal antibody (21079-1-AP)

    IF Staining of HepG2 using 21079-1-AP

    Immunofluorescent analysis of HepG2 cells, using FAM119A antibody 21079-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inK-562 cells, L02 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21079-1-AP is FAM119A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM119A fusion protein Ag15282
Full Name family with sequence similarity 119, member A
Calculated Molecular Weight 218 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC033720
Gene Symbol FAM119A
Gene ID (NCBI) 151194
RRIDAB_10755289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM119A antibody 21079-1-APDownload protocol
IHC protocol for FAM119A antibody 21079-1-APDownload protocol
IF protocol for FAM119A antibody 21079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Biol Chem

Lysine trimethylation regulates 78 kDa glucose-regulated protein proteostasis during endoplasmic reticulum stress.

Authors - Jonas Sieber
View Article
humanWB

J Proteomics

Comparative proteomic analysis of protein methylation provides insight into the resistance of hepatocellular carcinoma to 5-fluorouracil.

Authors - Zhen Liu
View Article