• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FAM175B Polyclonal antibody

FAM175B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26868-1-AP

Synonyms

Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HL-60 cells using FAM175B Polyclonal antibody (26868-1-AP)

    WB analysis of HL-60 using 26868-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FAM175B Polyclonal antibody (26868-1-AP)

    WB analysis of HEK-293 using 26868-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FAM175B Polyclonal antibody (26868-1-AP)

    WB analysis of mouse heart using 26868-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using FAM175B Polyclonal antibody (26868-1-AP)

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using FAM175B Polyclonal antibody (26868-1-AP)

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, HEK-293 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26868-1-AP is FAM175B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM175B fusion protein Ag22121
Full Name family with sequence similarity 175, member B
Calculated Molecular Weight 415 aa, 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008999
Gene Symbol FAM175B
Gene ID (NCBI) 23172
RRIDAB_2880663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM175B antibody 26868-1-APDownload protocol
IHC protocol for FAM175B antibody 26868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols