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  • KD/KO Validated

FAM84B Polyclonal antibody

FAM84B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18421-1-AP

Synonyms

FAM84B, NSE2, Protein FAM84B, Protein NSE2



Tested Applications

Positive WB detected inMDA-MB-453s cells, Jurkat cells
Positive IP detected inHUVEC cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18421-1-AP targets FAM84B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM84B fusion protein Ag13243
Full Name family with sequence similarity 84, member B
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC052957
Gene Symbol FAM84B
Gene ID (NCBI) 157638
RRIDAB_2278161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM84B antibody 18421-1-APDownload protocol
IHC protocol for FAM84B antibody 18421-1-APDownload protocol
IP protocol for FAM84B antibody 18421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Genet

Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.

Authors - Zheng Hu
humanWB,IHC

Gigascience

Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.

Authors - Caixia Cheng
  • KD Validated
humanIF

Proc Natl Acad Sci U S A

An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.

Authors - Yan Huang
  • KD Validated
humanWB,IHC

Ther Adv Med Oncol

FAM84B promotes prostate tumorigenesis through a network alteration.

Authors - Yanzhi Jiang
humanIHC

Aging (Albany NY)

FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway.

Authors - Xin Zhang
human,mouseWB,IHC

Oncotarget

Upregulation of FAM84B during prostate cancer progression.

Authors - Nicholas Wong