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FANCC Polyclonal antibody

FANCC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16973-1-AP

Synonyms

FA3, FAC, FACC, FANCC, Fanconi anemia group C protein, Protein FACC

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FANCC Polyclonal antibody (16973-1-AP)

    WB analysis of HepG2 using 16973-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FANCC Polyclonal antibody (16973-1-AP)

    WB analysis using 16973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FANCC Polyclonal antibody (16973-1-AP)

    WB analysis of K-562 using 16973-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FANCC Polyclonal antibody (16973-1-AP)

    WB analysis of Jurkat using 16973-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using FANCC Polyclonal antibody (16973-1-AP)

    IHC staining of human brain using 16973-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FANCC Polyclonal antibody (16973-1-AP)

    IHC staining of human brain using 16973-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, K-562 cells, Jurkat cells, NIH/3T3 tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16973-1-AP targets FANCC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANCC fusion protein Ag9423
Full Name Fanconi anemia, complementation group C
Calculated Molecular Weight 558 aa, 63 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC015748
Gene Symbol FANCC
Gene ID (NCBI) 2176
RRIDAB_2231524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FANCC antibody 16973-1-APDownload protocol
IHC protocol for FANCC antibody 16973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols