FARSB Polyclonal antibody

FARSB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16341-1-AP

Synonyms

FARSB, FARSLB, FRSB, HSPC173, PheHB, PheRS



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HL-60 cells, K-562 cells, human liver tissue, mouse brain tissue, PC-3 cells, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16341-1-AP targets FARSB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FARSB fusion protein Ag9522
Full Name phenylalanyl-tRNA synthetase, beta subunit
Calculated Molecular Weight 589 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC017783
Gene Symbol FARSB
Gene ID (NCBI) 10056
RRIDAB_2102515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FARSB antibody 16341-1-APDownload protocol
IHC protocol for FARSB antibody 16341-1-APDownload protocol
IF protocol for FARSB antibody 16341-1-APDownload protocol
IP protocol for FARSB antibody 16341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
ratWB

Neural Regen Res

A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases.

Authors - Byung Sun Park
human,mouseWB,IF

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
human,mouseWB,IHC,IF

Int J Mol Sci

FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway

Authors - Yaofeng Wang